Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High resolution dual laser flow cytometry.

P N Dean, D Pinkel

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |August 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Response to letter by Pulsipher et al.

    Leukemia·2010
    Same author

    Cell sizing: a small-angle light-scattering method for sizing particles of low relative refractive index.

    Applied optics·2010
    Same author

    'Allogeneic marrow transplantation in children with acute leukemia: a practice whose time has gone': twenty years later.

    Leukemia·2009
    Same author

    Confocal microscopy: principles and practices.

    Current protocols in cytometry·2008
    Same author

    Comparative genomic hybridization.

    Current protocols in human genetics·2008
    Same author

    Novel risk stratification of patients with neuroblastoma by genomic signature, which is independent of molecular signature.

    Oncogene·2007
    Same journal

    Expression and Spatial Localization of Glycosaminoglycan Types in Cartilage From Different Anatomical Locations.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    Aquaporin-4 Localization in Terminal Glial Cells of Human Mechanosensory Corpuscles: An Immunohistochemical Study of Penile Tissues.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    Same journal

    From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
    See all related articles

    This study introduces a high-resolution flow cytometer with sequential dual-laser excitation, enabling independent dye analysis in double staining experiments. This innovation overcomes limitations of single-illumination instruments, expanding dye combination possibilities.

    Area of Science:

    • Biotechnology
    • Cellular Biology
    • Optical Physics

    Background:

    • Flow cytometers use light sources and fluorescent dyes to analyze cellular components.
    • Existing single-illumination instruments have limitations in analyzing multiple fluorescent dyes simultaneously due to spectral overlap.

    Purpose of the Study:

    • To develop a high-resolution flow cytometer capable of independent excitation of two fluorescent dyes for double staining.
    • To overcome spectral overlap limitations in flow cytometry analysis.

    Main Methods:

    • Constructed a flow cytometer utilizing two spatially separated laser beams for sequential excitation of fluorescent dyes.
    • Implemented new light collection optics allowing up to four measurements per cell.
    • Sequential excitation with a 20-microsecond time separation between laser beams.

    Related Experiment Videos

    Main Results:

    • Achieved independent excitation of two dyes, allowing sequential emission analysis.
    • Demonstrated that spectral overlap of emissions does not impede analysis due to temporal separation.
    • Enabled up to four measurements per cell, significantly increasing usable dye combinations.

    Conclusions:

    • The developed sequential dual-laser excitation flow cytometer effectively resolves limitations of single-illumination systems.
    • This advancement broadens the scope of dye combinations applicable in flow cytometry, enhancing analytical capabilities.