Density-Based High-Quality Fat: Characterization and Correlation with Different Body Fat Ratio.

Area of Science:

• Regenerative Medicine
• Plastic Surgery
• Biomaterials Science

Background:

• Coleman's centrifugation method separates lipoaspirate into high-quality fat (HQF) and low-quality fat using a density float.
• The proportion of HQF varies significantly among individuals.

Purpose of the Study:

• To investigate the characteristics of HQF from different donors.
• To determine the relationship between donor body composition and HQF yield.
• To develop a predictive model for lipoaspirate volume in fat grafting procedures.

Main Methods:

• Lipoaspirate processed using Coleman's centrifugation with a 0.935 g/ml density float.
• Analysis of fat characteristics and in vivo grafting of HQF.
• Correlation analysis between HQF percentage and donor body fat ratio.

Main Results:

• HQF exhibited consistent stromal vascular fraction cell counts and extracellular matrix content across donors.
• In vivo studies showed similar graft appearance, retention, and histological outcomes for HQF at 12 weeks.
• A negative correlation was found between HQF percentage and donor body fat ratio.

Conclusions:

• HQF derived from various donors demonstrates uniform characteristics and transplantation efficacy.
• Donor body fat percentage is a key factor influencing HQF yield.
• A novel formula can predict required lipoaspirate volume, enhancing fat grafting predictability.