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Updated: Sep 6, 2025
Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
Published on: February 1, 2022
Stimulated Raman histology facilitates accurate diagnosis in neurosurgical patients: a one-to-one noninferiority
Evan H Einstein1, Faina Ablyazova2, Ashley Rosenberg2
1Department of Neurosurgery, Lenox Hill Hospital/Donald, Barbara Zucker School of Medicine at Hofstra, New York, NY, USA. eeinstein@northwell.edu.
Objective:
Stimulated Raman histology (SRH) offers efficient and accurate intraoperative neuropathological tissue analysis without procedural alteration to the diagnostic specimen. However, there are limited data demonstrating one-to-one tissue comparisons between SRH and traditional frozen sectioning. This study explores the non-inferiority of SRH as compared to frozen section on the same piece of tissue in neurosurgical patients.
Methods:
Tissue was collected over a 1-month period from 18 patients who underwent resection of central nervous system lesions. SRH and frozen section analyses were compared for diagnostic capabilities as well as assessed for quality and condition of tissue via a survey completed by pathologists.
Results:
SRH was sufficient for diagnosis in 78% of specimens as compared to 94% of specimens by frozen section of the same specimen. A Fisher's exact test determined there was no significant difference in diagnostic capability between the two groups. Additionally, both quality of SRH and condition of tissue after SRH were deemed to be non-inferior to frozen section.
Conclusions:
This study provides further evidence for the non-inferiority of SRH techniques. It is also the first study to demonstrate SRH accuracy using one-to-one tissue analysis in neuropathological specimens.
