Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Brendan Le Daré, Aurélien Couette, Romain Pelletier

    Annales De Biologie Clinique
    |June 29, 2022
    PubMed
    Summary

    Alpha- and beta-amanitin mushroom toxins cause severe liver damage. This review details methods for detecting amanitins in biological fluids, crucial for diagnosing and managing poisoning cases.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of bladder wash for postmortem toxicological analysis: Comparison with urine in 50 forensic cases.

    International journal of legal medicine·2026
    Same author

    N-Ethylpentedrone: first hair detection and human metabolic profiling across multiple biological matrices using UHPLC-HRMS, molecular networking and in silico prediction.

    Archives of toxicology·2026
    Same author

    Lentiviral-mediated expression of cytochrome P450 2D6 in HepaRG cells: new means for in vitro xenobiotic biotransformation and cellular response to chimeric viral mRNA.

    Archives of toxicology·2026
    Same author

    Optimization of fecal near infrared spectroscopy for predicting organic matter digestibility in cows using local algorithms.

    Journal of animal science·2026
    Same author

    Influence of hepatic steatosis on antiseizure drug metabolism: in vitro evidence from differentiated HepaRG cells.

    Toxicology letters·2026
    Same author

    Mapping the human chemical exposome for public health.

    Nature medicine·2026

    Area of Science:

    • Toxicology
    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Alpha- and beta-amanitin are potent toxins from higher fungi, causing severe, life-threatening liver injury.
    • Increasing mushroom poisonings reported in France necessitate robust biological diagnostic methods.
    • Accurate detection of amanitins in blood or urine is vital for patient management in hospital toxicology.

    Purpose of the Study:

    • To conduct a mini-literature review on quantifying amanitins in biological fluids for diagnosing amanitin poisoning.
    • To present characteristics of amanitins, analytical methods, interpretation data, and practical applications.
    • To compare two liquid chromatography-tandem mass spectrometry techniques and share practical experience.

    Main Methods:

    • Literature review on amanitin detection methods.
    • Comparison of Waters Xevo TQ XS and Thermo Scientific Q Exactive tandem mass spectrometry systems.
    • Analysis of biological samples (blood, urine) for amanitin quantification.

    Main Results:

    • Amanitin detection in biological fluids is essential for diagnosing poisoning.
    • Liquid chromatography-tandem mass spectrometry offers robust quantification methods.
    • Continuous improvement of analytical methods enhances diagnostic accuracy and patient care.

    Conclusions:

    • Reliable detection of amanitins in biological samples is critical for timely diagnosis and treatment of poisoning.
    • Comparative analysis of LC-MS/MS techniques aids in method optimization.
    • Ongoing refinement of analytical methodologies improves the management of amanitin toxicity.

    Related Experiment Videos