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Dwarf polish wheat hosts a novel closterovirus: Revelation by transcriptome data-mining.

Venkidusamy Kavi Sidharthan, Virendra Kumar Baranwal

    Acta Virologica
    |June 29, 2022
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    A novel closterovirus, Triticum polonicum closterovirus (TriPCV), was identified in dwarf polish wheat using public transcriptome data. Phylogenetic analysis confirms TriPCV as a putative new member of the Closterovirus genus.

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    Area of Science:

    • Plant Virology
    • Molecular Biology
    • Bioinformatics

    Background:

    • Closteroviruses are positive-sense single-stranded RNA viruses known for their narrow host range and widespread distribution.
    • Understanding closterovirus diversity is crucial for plant disease management and agricultural productivity.

    Purpose of the Study:

    • To identify and characterize a putative novel closterovirus from dwarf polish wheat.
    • To determine the phylogenetic relationship of the novel virus to existing closteroviruses.

    Main Methods:

    • Bioinformatic analysis of publicly available transcriptome data from dwarf polish wheat.
    • Genome assembly and annotation of the putative novel closterovirus.
    • Phylogenetic analysis based on viral genome sequences and protein domains.

    Main Results:

    • Identification of a putative novel closterovirus, Triticum polonicum closterovirus (TriPCV), with a 15.36 kb genome.
    • The TriPCV genome contains nine open reading frames (ORFs) encoding proteins for replication, movement, encapsidation, and RNA silencing suppression.
    • Phylogenetic analysis indicates TriPCV is distantly related to other known Closterovirus members.

    Conclusions:

    • Triticum polonicum closterovirus (TriPCV) is proposed as a putative novel species within the genus Closterovirus.
    • The findings expand the known diversity of closteroviruses and highlight the utility of public domain data for virus discovery.