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Updated: Aug 11, 2026

Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 3, 2015
[Effect of alpha-thrombin on DNA synthesis and excretion by lymphocytes]
Abstract:
Thrombin-activated spleen lymphocytes of mice synthesize 1.5 times more DNA than unstimulated cells. The proportions of palindromic sequences in the newly-synthesized nuclear DNA of control and thrombin-stimulated lymphocytes are 1.1% and 0.5%, respectively. Both the control and thrombin-stimulated lymphocytes release about 10-12% of the newly-synthesized cellular DNA into the culture medium. It is shown that the newly-synthesized DNA of viable, rather than non-viable cells, is the source of extracellular DNA.
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