Exploring solid-phase proximity ligation assay for survivin detection in urine.

Area of Science:

• Oncology
• Biochemistry
• Analytical Chemistry

Background:

• Urine biomarkers offer a non-invasive approach for bladder cancer detection.
• Survivin is a well-researched biomarker for bladder cancer.
• Accurate detection of low survivin levels is challenging.

Purpose of the Study:

• To develop a highly sensitive assay for urinary survivin detection.
• To evaluate the diagnostic performance of the survivin assay for bladder cancer.
• To assess survivin as a complementary biomarker for bladder cancer detection.

Main Methods:

• Development of a solid-phase proximity ligation assay (spPLA) for survivin detection.
• Utilized magnetic beads for molecular isolation of survivin from urine.
• Assay designed for small urine volumes (500 μL).

Main Results:

• The survivin spPLA achieved a limit of detection of 1.45 pg/mL.
• The assay demonstrated 30% sensitivity and 89% specificity for bladder cancer.
• Combining survivin spPLA with UBC® Rapid increased sensitivity to 70% (86% specificity).

Conclusions:

• The developed survivin spPLA is a sensitive tool for detecting low concentrations of survivin in complex biological matrices.
• Survivin shows potential as a complementary biomarker for bladder cancer detection when used with other markers.
• The assay may advance survivin biomarker research in various human fluids.