Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

1.8K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
1.8K
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

3.0K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell transcriptome reveals keratinocyte subclusters contributing to altered differentiation and inflammatory responses in atopic dermatitis.

Nature communications·2026
Same author

Gas-phase unfolding assay rapidly predicts structure-function relationships in engineered antibodies with tuned flexibilities.

Nature communications·2026
Same author

Transcriptomic Profiling of Cutaneous Melanoma Metastases Treated With Microwave Ablation-Pilot Study.

Clinical and translational science·2026
Same author

Immunopeptidomics-guided identification of functional neoantigens in non-small cell lung cancer.

NPJ precision oncology·2026
Same author

Integrated multiomic profiling reveals 2 distinct splenic marginal zone lymphoma subgroups with prognostic relevance.

Blood advances·2026
Same author

Realizing the potential of agonistic antibody immunotherapy.

Nature reviews. Drug discovery·2026

Related Experiment Video

Updated: Sep 6, 2025

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
05:24

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Published on: February 21, 2021

4.3K

KIR2DS2 Expression Identifies NK Cells With Enhanced Anticancer Activity.

Matthew D Blunt1, Andres Vallejo Pulido2, Jack G Fisher2

  • 1School of Clinical and Experimental Sciences, University of Southampton, Southampton, United Kingdom; and m.d.blunt@soton.ac.uk.

Journal of Immunology (Baltimore, Md. : 1950)
|June 29, 2022
PubMed
Summary

Killer cell Ig-like receptors (KIRs) like KIR2DS2 enhance natural killer (NK) cell anticancer activity. This research shows KIR2DS2 marks NK cells primed for improved cancer immunotherapy outcomes.

More Related Videos

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells
11:08

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Published on: February 14, 2025

1.0K
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
08:17

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

21.2K

Related Experiment Videos

Last Updated: Sep 6, 2025

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia
05:24

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Published on: February 21, 2021

4.3K
Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells
11:08

Advances in Human Induced Pluripotent Stem Cell-Derived Chimeric Antigen Receptor-Expressing Natural Killer Cells

Published on: February 14, 2025

1.0K
Flow Cytometry-based Assay for the Monitoring of NK Cell Functions
08:17

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

21.2K

Area of Science:

  • Immunology
  • Cancer Biology
  • Cellular Therapeutics

Background:

  • Natural killer (NK) cells are crucial for cancer immunity.
  • Activating killer cell Ig-like receptors (KIRs) influence NK cell function and cancer outcomes.
  • KIR2DS2 shows promise in hematological and solid tumors, but its mechanism is unclear.

Purpose of the Study:

  • To investigate the impact of KIR2DS2 expression on NK cell function.
  • To elucidate the mechanisms underlying KIR2DS2's association with improved cancer outcomes.
  • To identify KIR2DS2 as a potential target for NK cell-based cancer therapies.

Main Methods:

  • Novel flow cytometry panel to analyze KIR2DS2 expression on NK cells.
  • Assessment of NK cell activation against various cancer cell lines and primary leukemia cells.
  • Bulk and single-cell RNA sequencing to identify molecular pathways associated with KIR2DS2 expression.

Main Results:

  • High KIR2DS2 expression correlates with enhanced spontaneous NK cell activation against B cell lines, liver cancer, and chronic lymphocytic leukemia.
  • KIR2DS2high NK cells exhibit increased CD16 expression and augmented antibody-dependent cellular cytotoxicity (ADCC).
  • RNA sequencing reveals upregulation of cytotoxicity, translation, and FCGR pathways in KIR2DS2high NK cells.

Conclusions:

  • KIR2DS2 marks a subset of NK cells with heightened anticancer activity.
  • KIR2DS2 expression is linked to enhanced NK cell cytotoxicity and response to antibody-based therapies.
  • KIR2DS2 represents a promising target for developing novel NK cell-based cancer immunotherapies.