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MSCs-Derived miR-150-5p-Expressing Exosomes Promote Skin Wound Healing by Activating PI3K/AKT Pathway through PTEN

Cheng Xiu1, Huining Zheng1, Manfei Jiang1

  • 1Department of Plastic Surgery and Medical Cosmetology, Hainan Cancer Hospital, Haikou, Hainan, China.

International Journal of Stem Cells
|June 30, 2022
PubMed
Abstract

Insights

Mesenchymal stem cell-derived exosomes carrying microRNA-150-5p promote skin wound healing. These exosomes activate the PI3K/AKT pathway by targeting PTEN, enhancing cell growth and migration while reducing apoptosis.

Area of Science:

  • Regenerative Medicine
  • Molecular Biology
  • Cell Biology

Background:

  • Skin wound healing is a complex process influenced by various cellular and molecular factors.
  • Mesenchymal stem cells (MSCs) and their secreted exosomes show therapeutic potential in tissue repair.
  • MicroRNAs (miRNAs) are key regulators of gene expression involved in cellular processes like wound healing.

Purpose of the Study:

  • To investigate the mechanism by which MSC-derived exosomal miR-150-5p promotes skin wound healing.
  • To elucidate the role of the PI3K/AKT signaling pathway and PTEN in this process.
  • To determine if miR-150-5p directly targets PTEN.

Main Methods:

  • Human umbilical cord MSCs (HUC-MSCs) were engineered to overexpress miR-150-5p and their exosomes (MSCs-Exos) were isolated.
  • A cellular model of skin injury was established using H2O2-treated HaCaT cells.
  • Cell proliferation, migration, and apoptosis were assessed, along with PTEN expression and PI3K/AKT pathway activation.
  • Bioinformatic tools, dual-luciferase reporter assays, and RIP assays were used to confirm the interaction between miR-150-5p and PTEN.

Main Results:

  • H2O2-induced HaCaT cells showed decreased miR-150-5p and increased PTEN expression.
  • MSCs-Exos promoted HaCaT cell proliferation and migration while inhibiting apoptosis.
  • Overexpression of exosomal miR-150-5p enhanced these protective effects.
  • PTEN was identified as a direct target of miR-150-5p, and miR-150-5p regulated the PI3K/AKT pathway via PTEN.

Conclusions:

  • MSC-derived exosomes overexpressing miR-150-5p significantly enhance skin wound healing.
  • The mechanism involves the activation of the PI3K/AKT pathway through the downregulation of PTEN by miR-150-5p.
  • These findings highlight the therapeutic potential of exosomal miR-150-5p for skin regeneration.
Keywords:
ApoptosisMesenchymal stem cell-derived exosomesMigrationPI3K/AKT pathwayPTENProliferationSkin wound healingmiR-150-5p

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