Related Experiment Video
Updated: Sep 6, 2025
In Vitro 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow
Published on: March 14, 2021
Vasculogenic Mimicry in a 3D Model In Vitro
Emily Marques Dos Reis1, Fernanda Vieira Berti2, Luismar Marques Porto2
1Chemical and Food Engineering Department, Federal University of Santa Catarina, Florianópolis, SC, Brazil. emimreis@gmail.com.
Abstract:
Increasing studies on vasculogenic mimicry (VM) have shown that the hypoxic microenvironment and the presence of endothelial cell play an important role in regulating tumor phenotype and aggressiveness. Thus, the representation of these factors in vitro becomes necessary to mimic VM. This chapter provides a protocol for mimicking VM in vitro in a more robust 3D model that includes the presence of 3D matrix, melanoma cells, a hypoxia-inducing agent, and endothelial cells.
More Related Videos
06:36An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors
Published on: May 13, 2019
08:04In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
Published on: May 11, 2021