Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry In Vitro

Vasculogenic mimicry formation is generally assessed using three-dimensional (3D) cultures of aggressive tumor cells grown over an extended incubation period. Test agents can be introduced during growth of the 3D cultures to determine their effect on vasculogenic mimicry formation. Here, we describe the protocol for evaluation of the inhibitory effect of drugs on vasculogenic mimicry in vitro using bright-field and fluorescence microscopy on 3D cultures of tumor cells grown in Matrigel.