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Updated: Sep 6, 2025
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Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
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Modeling Asthma in Mice Using Rhinovirus Infection.
1Departments of Pediatrics, University of Michigan Medical School, Ann Arbor, MI, USA. kbentley@umich.edu.
Methods in Molecular Biology (Clifton, N.J.)
|June 30, 2022
Summary
This study details methods for producing standardized rhinovirus (RV) and infecting mice. These methods enable researchers to study RV
Area of Science:
- Virology
- Immunology
- Respiratory Medicine
Background:
- Rhinovirus (RV) infection is a significant factor in early-life wheezing and adult asthma exacerbation.
- Animal models are crucial for understanding RV pathogenesis and developing therapeutic strategies.
Purpose of the Study:
- To provide standardized methods for human rhinovirus A1B (RV) production and mouse infection.
- To outline protocols for combining RV infection with allergen administration in mouse models.
Main Methods:
- Virus production involves amplification, purification, and concentration of human rhinovirus A1B.
- Standardization of RV stock is achieved through plaque assays on HeLa cells to determine plaque-forming units (PFUs).
- Standardized RV is then used for infection in adult and neonatal mouse models, with options for co-administration of allergens like ovalbumin and house dust mite.
Main Results:
- Established protocols for reproducible production of standardized human rhinovirus A1B.
- Demonstrated successful application of these methods for mouse infection models.
- Validated a plaque assay for accurate quantification of viral infectivity.
Conclusions:
- The described methods provide a robust framework for studying rhinovirus infection and its role in respiratory diseases using mouse models.
- Standardized viral preparations and infection protocols enhance the reliability and reproducibility of research findings in this field.
