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Updated: Sep 6, 2025

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
Published on: May 16, 2022
Dual-wavelength multimodal multiphoton microscope with SMA-based depth scanning
Wentao Wu1,2, Qihao Liu1, Christoph Brandt1
1Department of Electrical and Computer Engineering, University of British Columbia, 5500-2332 Main Mall, Vancouver, BC V6 T 1Z4, Canada.
Abstract:
We report on a multimodal multiphoton microscopy (MPM) system with depth scanning. The multimodal capability is realized by an Er-doped femtosecond fiber laser with dual output wavelengths of 1580 nm and 790 nm that are responsible for three-photon and two-photon excitation, respectively. A shape-memory-alloy (SMA) actuated miniaturized objective enables the depth scanning capability. Image stacks combined with two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG) signals have been acquired from animal, fungus, and plant tissue samples with a maximum depth range over 200 µm.
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