Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic analysis of Borrelia.

F W Hyde, R C Johnson

    Zentralblatt Fur Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology
    |December 1, 1986
    PubMed
    Summary

    DNA homology studies reveal genus-level relationships among Borrelia species and Ornithodoros coriaceus spirochetes. A unique B. burgdorferi plasmid found in North American ticks warrants further investigation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Host cell-specific expression of a p44 epitope by the human granulocytic ehrlichiosis agent.

    The Journal of infectious diseases·2001
    Same author

    Distinct roles for the two Rho GDP/GTP exchange factor domains of kalirin in regulation of neurite growth and neuronal morphology.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·2001
    Same author

    Vitamin-mineral supplementation and accelerated chilling effects on quality of pork from pigs that are monomutant or noncarriers of the halothane gene.

    Journal of animal science·2001
    Same author

    Molecular typing of the etiologic agent of human granulocytic ehrlichiosis.

    Journal of clinical microbiology·2001
    Same author

    How far do electrons move? A semiempirical investigation of thermal electron-transfer distances in cationic bis(hydrazine) and bis(hydrazyl) mixed-valence compounds.

    Journal of the American Chemical Society·2001
    Same author

    Nuclear localization of the Saccharomyces cerevisiae HMG protein NHP6A occurs by a Ran-independent nonclassical pathway.

    Traffic (Copenhagen, Denmark)·2001

    Area of Science:

    • Microbiology
    • Genetics
    • Molecular Biology

    Background:

    • Borrelia species are known tick-borne pathogens.
    • Understanding genetic relatedness is crucial for disease control.
    • Plasmid DNA's role in spirochete biology is often unclear.

    Purpose of the Study:

    • To determine the DNA homology among various Borrelia species and the Ornithodoros coriaceus spirochete.
    • To investigate the presence and homology of plasmid DNA within these spirochetes.
    • To characterize a specific plasmid found in Borrelia burgdorferi.

    Main Methods:

    • DNA homology studies using Southern blot analysis.
    • Plasmid DNA extraction and characterization.
    • Comparison of plasmid DNA sequences across different isolates.

    Main Results:

    • Significant DNA homology (17-53%) was observed among B. hermsii, B. duttoni, B. crocidurae, and O. coriaceus, indicating a genus-level relationship.
    • Plasmid DNA was detected in Borrelia strains, but showed little to no homology with B. hermsii plasmid DNA.
    • A 9.2 kilobase tandem repeat plasmid in B. burgdorferi strain CT1 was identified and found in North American tick isolates but not in European or other surveyed isolates.

    Conclusions:

    • Borrelia species and O. coriaceus spirochetes share genus-level genetic relatedness.
    • Plasmid DNA content varies among these spirochetes, with limited inter-strain homology.
    • A specific B. burgdorferi plasmid prevalent in North American ticks suggests potential geographic significance, though its biological function remains unknown.

    Related Experiment Videos