Prostasomes in human semen contain ADP and GDP

Prostasomes from human semen contained ADP and GDP (mean values 81 +/- 58 respective 71 +/- 46 nmoles/g protein; n = 20) while other nucleoside phosphates were not detected as determined by high performance liquid chromatography. A strong linear relationship (r = 0.80, p less than 0.001) existed between ADP and GDP in prostasomes, while ADP (but not GDP) correlated inversely with semen volume (r = 0.54, p less than 10.02). The possibility is discussed of ADP and GDP being incapsulated within the prostasomes as a macromolecular complex involving stacked nucleotides held together by divalent cations through the phosphate groups.