Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.2K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.2K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.4K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acupuncture promotes post-stroke angiogenesis and neuroprotection through regulation of the DLL4/Notch1 signaling pathway.

Histology and histopathology·2026
Same author

Curcumin ameliorates metabolic dysfunction-associated steatotic liver disease <i>via</i> targeting triokinase/FMN cyclase to regulate the expression of glycerol-3-phosphate acyltransferase 3: Integration of chemical proteomics and transcriptomics.

Acta pharmaceutica Sinica. B·2026
Same author

Corrigendum to 'Generation of potent cellular and humoral immunity against SARS-CoV-2 antigens via conjugation to a polymeric glyco-adjuvant' [Biomaterials, 128 (2021), 121159].

Biomaterials·2026
Same author

Harnessing a single molecule for dual bioorthogonal regulation of RNA function and m6A methylation.

Nucleic acids research·2026
Same author

Rapid identification of binary and ternary adulteration in camellia oil by CRISPR/Cas12a assay.

Food chemistry·2026
Same author

A Closed-Loop ta-VNS System Synchronized with BCI-Based Motor Training for Post-Stroke Upper Limb Rehabilitation.

Journal of visualized experiments : JoVE·2026

Related Experiment Video

Updated: Sep 6, 2025

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.5K

Fluorescence recovery based on synergetic effect for ALP detection.

Siqi Huang1, Weishan Yang2, Silu Ye1

  • 1CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics. Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|July 1, 2022
PubMed
Summary

A new method uses Cadmium-zinc-selenium (CdZnSe) quantum dots to detect alkaline phosphatase (ALP), a key biomarker. This approach offers sensitive and selective ALP detection in biological samples.

Keywords:
Alkaline phosphataseQuantum dotsSensorSynergetic fluorescence recoveryTurn-off–on

More Related Videos

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
07:00

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

11.3K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

Related Experiment Videos

Last Updated: Sep 6, 2025

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

5.5K
Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
07:00

Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

11.3K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Alkaline phosphatase (ALP) is a crucial enzyme and biomarker linked to various health conditions, including diabetes, liver disease, bone disorders, and breast cancer.
  • Accurate and sensitive detection of ALP is vital for early diagnosis and monitoring of these diseases.
  • Existing detection methods may lack sensitivity, selectivity, or require complex procedures.

Purpose of the Study:

  • To develop a novel, sensitive, and selective method for alkaline phosphatase (ALP) detection.
  • To utilize synergetic fluorescence recovery based on Cadmium-zinc-selenium (CdZnSe) quantum dots (QDs).
  • To establish a cost-effective and efficient detection strategy for ALP in biological samples.

Main Methods:

  • Synthesized Cadmium-zinc-selenium (CdZnSe) quantum dots (QDs) using a simple one-pot water bath method.
  • Investigated the fluorescence quenching mechanism of CdZnSe QDs by potassium permanganate (KMnO4) and ascorbic acid phosphate (AAP).
  • Exploited the ALP-catalyzed hydrolysis of AAP to ascorbic acid, which then reacts with KMnO4, inducing synergetic fluorescence recovery of CdZnSe QDs.

Main Results:

  • The developed method demonstrated a sensitive detection of ALP with a detection limit of 0.21 U/L in the concentration range of 2.5-250 U/L.
  • The synergetic fluorescence recovery efficiency showed a strong correlation with the logarithmic ALP concentration.
  • The method achieved good recovery rates when applied to ALP detection in human serum samples, indicating its practical applicability.

Conclusions:

  • The synergetic fluorescence recovery method using CdZnSe QDs offers a sensitive and selective approach for ALP detection.
  • The facile synthesis of CdZnSe QDs and the assay's performance highlight its potential for clinical diagnostics.
  • This study presents a novel strategy for enhancing detection sensitivity and selectivity of ALP, paving the way for improved biomarker analysis.