Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 6, 2025

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.4K

Understanding and evolving prions by yeast multiplexed assays.

Mireia Seuma1, Benedetta Bolognesi1

  • 1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri Reixac 10-12, 08028 Barcelona, Spain.

Current Opinion in Genetics & Development
|July 1, 2022
PubMed
Summary

Yeast genetics research reveals how mutations affect prion properties. New multiplexed deep mutagenesis strategies in yeast offer powerful insights into prion self-assembly and in vivo structure.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Massively parallel quantification of mutational impact on IAPP amyloid formation.

Nature communications·2026
Same author

Massively parallel genetic perturbation suggests the energetic structure of an amyloid-β transition state.

Science advances·2025
Same author

Massive mutagenesis reveals an incomplete amyloid motif in Bri2 that turns amyloidogenic upon C-terminal extension.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Massive experimental quantification allows interpretable deep learning of protein aggregation.

Science advances·2025
Same author

Massively parallel genetic perturbation reveals the energetic architecture of an amyloid beta nucleation reaction.

bioRxiv : the preprint server for biology·2024
Same author

Massive experimental quantification of amyloid nucleation allows interpretable deep learning of protein aggregation.

bioRxiv : the preprint server for biology·2024

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Yeast genetics has been pivotal in understanding prions, including their composition, conformation, and propagation.
  • Thirty years later, yeast remains a powerful model for studying prion sequence mutations and their effects.

Purpose of the Study:

  • To provide an overview of novel multiplexed strategies for investigating prions and prion-like sequences.
  • To explore the impact of mutations in prion sequences on their function, toxicity, and physical properties.

Main Methods:

  • Deep mutagenesis combined with tailored selection assays in yeast.
  • Mimicking evolutionary processes in vitro ('evolution in a flask').

Main Results:

More Related Videos

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
12:57

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans

Published on: January 8, 2015

16.1K
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
10:03

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

Published on: July 16, 2008

27.5K

Related Experiment Videos

Last Updated: Sep 6, 2025

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
08:12

High-throughput Screening for Protein-based Inheritance in S. cerevisiae

Published on: August 8, 2017

6.4K
Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
12:57

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans

Published on: January 8, 2015

16.1K
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
10:03

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

Published on: July 16, 2008

27.5K
  • These multiplexed approaches reveal mechanistic insights into the consequences of prion self-assembly.
  • The study reports on the structures that prion sequences adopt in vivo.

    • Conclusions:

    • Multiplexed deep mutagenesis in yeast is a powerful strategy for studying prions.
    • This approach enhances understanding of prion structure-function relationships and in vivo behavior.