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Developing shelf-stable Microbiota Directed Complementary Food (MDCF) prototypes for malnourished children: study

Ishita Mostafa1,2, Shah Mohammad Fahim3, Subhasish Das1,4

  • 1Nutrition and Clinical Services Division, icddr,b, Dhaka, 1212, Bangladesh.

BMC Pediatrics
|July 1, 2022
PubMed
Summary

Developing shelf-stable therapeutic foods for moderate acute malnutrition (MAM) is crucial. This study evaluates new formulations to ensure they effectively repair gut microbiota disruptions in malnourished children.

Keywords:
Gut MicrobiotaMicrobiota Directed Complementary FoodModerate Acute Malnutrition

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Area of Science:

  • Nutrition Science
  • Microbiome Research
  • Global Health

Background:

  • Childhood undernutrition, particularly moderate acute malnutrition (MAM), affects millions of children globally.
  • Current treatment lacks global guidelines, and existing interventions like MDCF-2 require daily preparation.
  • There's a need for shelf-stable, bioequivalent formulations of microbiota-directed complementary food (MDCF-2) to improve treatment accessibility.

Purpose of the Study:

  • To assess the acceptability and microbiota repair equivalence of scalable, shelf-stable formulations of MDCF-2.
  • To compare the efficacy of prototype shelf-stable MDCF formulations against the fresh MDCF-2 in Bangladeshi children with MAM.
  • To ensure new formulations support gut microbial community development in malnourished children.

Main Methods:

  • A randomized single-blind study in 8-12-month-old Bangladeshi children with MAM.
  • Comparison of shelf-stable MDCF prototypes with daily prepared MDCF-2.
  • Analysis of fecal DNA using V4-16S rDNA amplicon and shotgun sequencing to assess bacterial taxa abundance.
  • Equivalence defined by the absence of statistically significant differences in MDCF-2-responsive gut bacterial taxa after 4 weeks of treatment.

Main Results:

  • The study protocol is designed to evaluate the efficacy of shelf-stable formulations.
  • Microbiota repair will be assessed by changes in specific gut bacterial taxa.
  • Equivalence will be determined by comparing the microbial response to prototype formulations versus the fresh MDCF-2.

Conclusions:

  • This trial aims to establish the viability of shelf-stable therapeutic foods for MAM.
  • Successful development of these formulations could significantly improve treatment delivery and outcomes for malnourished children.
  • The findings will contribute to achieving global nutrition targets by providing accessible, effective treatments.