Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
RNA Interference01:23

RNA Interference

26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

7.9K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.9K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

7.8K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Protein Phosphatase Inhibitor LB100 Targets the Mesenchymal Lineage of Pancreatic Ductal Adenocarcinoma.

MedComm·2026
Same author

Dual FLT3/MAPK14 Proteolysis-Targeting Chimera (PROTAC) Induces Potent Acute Myeloid Leukemia Cell Death.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

The Host Cell Factor Phosphatase-2A Subunit PR130 Restricts Replication of Herpes Simplex Virus Type-1.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Dual inhibition of PP2A and WEE1 induces apoptosis and mitotic catastrophe in cancer cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

GSK3β<sup>high</sup>/NFATc1<sup>high</sup> subtype targeting overcomes therapy resistance in pancreatic cancer through transcriptional induction of homologous recombination repair.

Gut·2025
Same author

Identification of a Proteolysis-Targeting-Chimera that Addresses Activated Checkpoint Kinase-1 Reveals its Non-Catalytic Functions in Tumor Cells.

Angewandte Chemie (International ed. in English)·2025

Related Experiment Video

Updated: Sep 6, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

7.7K

RNA interference protocol to silence oncogenic drivers in leukemia cell lines.

Mandy Beyer1, Oliver H Krämer1

  • 1Institute of Toxicology, Johannes-Gutenberg University of Mainz, Obere Zahlbacher St. 67, 55131 Mainz, Germany.

STAR Protocols
|July 2, 2022
PubMed
Summary

This study presents an efficient method for silencing leukemia-associated proteins using small interfering RNAs (siRNAs) delivered via electroporation, maintaining high cell viability for functional studies.

Keywords:
CancerCell BiologyCell cultureCell-based AssaysMolecular Biology

More Related Videos

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

620
DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

20.7K

Related Experiment Videos

Last Updated: Sep 6, 2025

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

7.7K
Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

620
DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

20.7K

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Gene Silencing

Background:

  • Delivering small interfering RNAs (siRNAs) to leukemia cells for gene silencing is challenging.
  • High transfection efficiency and cell viability are critical for functional studies of leukemia-associated proteins.

Purpose of the Study:

  • To describe an efficient protocol for silencing oncogenic feline McDonough sarcoma (FMS)-like tyrosine kinase-3 in leukemia cells.
  • To provide a generally applicable method for decreasing RNA levels of proteins of interest in leukemia cells.

Main Methods:

  • Utilized small interfering RNAs (siRNAs) for genetic silencing.
  • Employed electroporation as the delivery method for siRNAs into leukemia cells.
  • Focused on silencing feline McDonough sarcoma (FMS)-like tyrosine kinase-3.

Main Results:

  • Achieved efficient siRNA delivery to leukemia cells.
  • Maintained high cell viability throughout the transfection process.
  • Successfully demonstrated the silencing of target oncogenic proteins.

Conclusions:

  • The described electroporation protocol is an effective strategy for siRNA delivery in leukemia cells.
  • This method facilitates functional studies of leukemia-associated proteins by enabling efficient gene silencing.
  • The protocol is adaptable for silencing various RNAs encoding proteins of interest in cancer research.