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Updated: Sep 6, 2025
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Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Published on: January 7, 2016
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Prolactin response to growth hormone stimulation tests.
Avivah Silbergeld1, Chen Barazani2, Zvi Laron1
1Endocrinology and Diabetes Research Unit, Schneider Children's Medical Centre, Petach Tikva, Israel.
Summary
Growth Hormone (GH) stimulation tests reveal distinct Prolactin (PRL) responses. Glucagon stimulates both GH and PRL, while Clonidine suppresses PRL and is superior for GH stimulation in children with short stature.
Area of Science:
- Pediatric Endocrinology
- Hormone Physiology
- Growth Disorders
Background:
- Prolactin (PRL) and Growth Hormone (GH) share structural and functional similarities.
- The clinical relationship between PRL and GH, particularly in response to stimulation tests, is not well understood.
Purpose of the Study:
- To investigate the Prolactin (PRL) response during Growth Hormone (GH) stimulation tests in children.
- To compare the effects of different GH stimulation agents on both GH and PRL secretion.
Main Methods:
- Retrospective analysis of data from 1083 prepubertal and early pubertal children with short stature.
- GH and PRL levels were measured by radioimmunoassay during stimulation tests using glucagon, clonidine, or clonidine with arginine.
- Peak GH and PRL levels were compared across different stimulation protocols.
Main Results:
- Glucagon stimulated both GH and PRL to similar levels.
- Clonidine, alone or with arginine, suppressed PRL secretion significantly (p < 0.0001).
- Clonidine-based tests were more effective for GH stimulation than glucagon, with higher GH levels observed during clonidine plus arginine, potentially related to pubertal stage.
Conclusions:
- This study elucidates the differential effects of common GH stimulation agents on PRL secretion in children.
- Findings highlight the complex interplay between GH and PRL regulation during endocrine testing.
- Provides valuable insights into the Prolactin-Growth hormone relationship in pediatric short stature.
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