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Updated: Sep 6, 2025

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology
Published on: January 26, 2024
Huge discrepancy between serum immunoglobulin concentration and proteinemia due to heavy chain disease
Guillaume Feugray1, Camille Fontaine2, Hélène Lanic3
1Department of General Biochemistry, F-76000 Rouen, France, Normandie Univ, UNIROUEN, INSERMU1096 EnVI.
Abstract:
Immunoassays are widely used in clinical laboratories because of their ease of use and low cost. These tests are based on antigen-antibody binding. However, clinicians and laboratory personnel may be confronted with immunoassay interference leading to difficulties in medical care. Here, we report a huge analytical discrepancy with IgG concentration higher than proteinemia in a 75-year-old man. Serum electrophoresis and immunofixation diagnosed γ-heavy chain disease. After investigation by different methods, the assay discrepancy was still present. We hypothesize that the interference is related to the truncated immunoglobulin secreted by the lymphoproliferative disorder.
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