Nose-only exposure system for inhalation exposures of rodents to large particles

A large-particle exposure system for small animals was designed, constructed and evaluated. The system was designed by incorporating a fluidized bed aerosol generator (FBG) and a nose-only exposure device to accommodate 40 small animals into a single unit. The system has four levels of exposure ports, each level having ten exposure ports radially positioned around the aerosol delivery components of the system. The aerosol generator produces aerosols that travel to the top of the system then downwards in order to be drawn past each animal's nose via vacuum ports immediately above the exposure ports. Nearly monodisperse polystyrene latex aerosols with nominal sizes of 3.0, 9.0 and 15.0 micron were generated as dry powders in an FBG with an inside diameter of 5 cm. During 60-min test runs, average aerosol mass concentrations up to 37 mg/m3 were achieved with less than 10% variation in mass concentration distribution throughout the unit.