Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gut microbiota promotes pain chronicity in Myosin1A deficient male mice.

Brain, behavior, and immunity·2024

Same author

Mapping the neuroethological signatures of pain, analgesia, and recovery in mice.

Neuron·2023

Same author

Taking a deep breath: How a brainstem pathway integrates pain and breathing.

Neuron·2022

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 6, 2025

Dynamic Quantitative Sensory Testing to Characterize Central Pain Processing

Dynamic Quantitative Sensory Testing to Characterize Central Pain Processing

Published on: February 16, 2017

Next generation behavioral sequencing for advancing pain quantification.

Z Anissa Jhumka¹, Ishmail J Abdus-Saboor²

¹Zuckerman Mind Brain Behavior Institute and Department of Biological Sciences, Columbia University, New York, NY, USA. Electronic address: https://twitter.com/AnissaJhumka.

Current Opinion in Neurobiology

|July 5, 2022

Summary

This summary is machine-generated.

Next-generation behavioral sequencing uses advanced videography and computation to precisely quantify rodent pain behaviors. This breakthrough offers a more sensitive and objective approach to understanding chronic pain and developing new analgesics.

More Related Videos

A Quantitative Sensory Testing Paradigm to Obtain Measures of Pain Processing in Patients Undergoing Breast Cancer Surgery

A Quantitative Sensory Testing Paradigm to Obtain Measures of Pain Processing in Patients Undergoing Breast Cancer Surgery

Published on: January 18, 2018

Investigating Pain-Related Avoidance Behavior using a Robotic Arm-Reaching Paradigm

Investigating Pain-Related Avoidance Behavior using a Robotic Arm-Reaching Paradigm

Published on: October 3, 2020

Related Experiment Videos

Last Updated: Sep 6, 2025

Dynamic Quantitative Sensory Testing to Characterize Central Pain Processing

Dynamic Quantitative Sensory Testing to Characterize Central Pain Processing

Published on: February 16, 2017

A Quantitative Sensory Testing Paradigm to Obtain Measures of Pain Processing in Patients Undergoing Breast Cancer Surgery

A Quantitative Sensory Testing Paradigm to Obtain Measures of Pain Processing in Patients Undergoing Breast Cancer Surgery

Published on: January 18, 2018

Investigating Pain-Related Avoidance Behavior using a Robotic Arm-Reaching Paradigm

Investigating Pain-Related Avoidance Behavior using a Robotic Arm-Reaching Paradigm

Published on: October 3, 2020

Area of Science:

Neuroscience
Animal Behavior
Pain Research

Background:

Chronic pain affects 20% of physician visits, with many patients dissatisfied with current treatments.
Rodent behavioral studies are crucial for pain research but often lack detailed characterization.
Existing methods may alter animal sensitivity or provide incomplete behavioral data.

Purpose of the Study:

To introduce and summarize next-generation behavioral sequencing for pain research.
To highlight the potential of advanced videography and computational approaches.
To improve the quantitative description of pain-related behaviors in animal models.

Main Methods:

Utilizing high-resolution videography to capture animal movements.
Employing computational algorithms for detailed behavioral analysis.
Focusing on millisecond to minutes long behavioral timescales.

Main Results:

Next-generation behavioral sequencing enables quantitative description of subtle behaviors.
These methods offer enhanced sensitivity beyond unaided observation.
The approach allows for more comprehensive characterization of pain phenotypes.

Conclusions:

Advanced behavioral analysis techniques can significantly improve pain research.
Next-generation sequencing offers a more objective and detailed understanding of pain processing.
This methodology holds promise for the development of novel analgesics and pain therapies.