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First morning voided urinary gonadotropins in children: verification of method performance and establishment of

Yifan Yao1, Shunfeng Mao2, Ke Yuan3

  • 1Department of Laboratory Medicine, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, P.R. China.

Clinical Chemistry and Laboratory Medicine
|July 5, 2022
PubMed

Insights

This study establishes reference intervals for urinary luteinizing hormone (uLH) and urinary follicle-stimulating hormone (uFSH) in children aged 6-11 years. These findings provide a valuable non-invasive tool for screening and managing central precocious puberty.

Area of Science:

  • Pediatric Endocrinology
  • Clinical Chemistry
  • Reproductive Biology

Background:

  • Urinary luteinizing hormone (uLH) and urinary follicle-stimulating hormone (uFSH) are crucial for diagnosing central precocious puberty.
  • Establishing reliable reference intervals for these urinary hormones is essential for accurate clinical application.
  • Existing studies on uLH and uFSH reference intervals in children are limited.

Purpose of the Study:

  • To establish age-, sex-, and pubertal status-specific reference intervals for uLH and uFSH.
  • To validate the analytical performance of uLH and uFSH assays for clinical use.
  • To provide a non-invasive screening tool for central precocious puberty in children.

Main Methods:

  • Assessed method performance including detection capability, precision, accuracy, linearity, and stability for uLH and uFSH.
  • Utilized Clinical Laboratory Standards Institute's C28-A3 criteria to establish reference intervals.
  • Correlated urinary hormone concentrations with serum levels to ensure validity.

Main Results:

  • Both uLH and uFSH demonstrated acceptable stability and measurement performance.
  • Recovery rates for uLH and uFSH were within acceptable limits (87.6-98.8% and 102.8-103.4%).
  • Significant correlations were observed between serum and urinary hormone concentrations (LH: r=0.91, FSH: r=0.90).

Conclusions:

  • Reference intervals for uLH and uFSH were successfully established based on age, sex, and pubertal status.
  • These established intervals offer a non-invasive method for the clinical screening of precocious puberty in children aged 6-11 years.
  • The findings support the utility of uLH and uFSH as valuable diagnostic tools in pediatric endocrinology.
Abstract

Keywords:
follicle-stimulating hormoneluteinizing hormoneprecocious pubertyreference interval

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