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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
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Diagnosis of Pancreatic Cancer Using miRNA30e Biosensor.

Namita Sharma1, Sudha Srivastava2

  • 1Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, UP, India.

Interdisciplinary Sciences, Computational Life Sciences
|July 5, 2022
PubMed
Summary

This study presents a novel electrochemical biosensor for detecting microRNA30e (miRNA30e), a pancreatic cancer biomarker. The developed biosensor offers highly sensitive detection, crucial for early cancer diagnosis and improved patient survival rates.

Keywords:
BiosensorElectrochemical impedance spectroscopymiR30e

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Pancreatic cancer diagnosis relies on invasive and time-consuming methods.
  • MicroRNAs (miRNAs) are emerging biomarkers for early cancer detection.
  • Development of sensitive and rapid diagnostic tools is critical.

Purpose of the Study:

  • To develop and validate an electrochemical biosensor for the sensitive detection of miRNA30e.
  • To utilize gold nanorods and DNA probes for enhanced miRNA detection.
  • To evaluate the biosensor's performance for potential early pancreatic cancer diagnosis.

Main Methods:

  • Functionalization of screen-printed gold electrodes with cysteine hydrochloride and gold nanorods.
  • Immobilization of single-stranded DNA probes specific to miRNA30e.
  • Electrochemical measurements using cyclic voltammetry (CV) and differential pulse voltammetry (DPV).
  • Analysis of electrochemical impedance spectroscopy (EIS) for sensitivity assessment.

Main Results:

  • The electrochemical biosensor demonstrated a wide detection range for miRNA30e from 0.1 fg/mL to 0.1 µg/mL (14.9 aM to 14.9 nM).
  • Differential pulse voltammetry (DPV) offered higher sensitivity (104.4 µA/µg/mL/cm²) compared to cyclic voltammetry (CV) (79.6 µA/µg/mL/cm²).
  • A very low limit of detection of 14.9 aM for miRNA30e was achieved.

Conclusions:

  • The developed miRNA-based electrochemical biosensor is a promising tool for sensitive and early detection of pancreatic cancer biomarkers.
  • This platform offers a potential alternative to conventional diagnostic methods, improving efficiency and reducing invasiveness.
  • The high sensitivity and low detection limit contribute to the potential for early cancer diagnosis and improved patient outcomes.