Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Altered lymphocyte subsets during cardiopulmonary bypass.

J I Brody, N J Pickering, G B Fink

    American Journal of Clinical Pathology
    |May 1, 1987
    PubMed
    Summary

    Open heart surgery temporarily reduces key immune cells (T4 helper lymphocytes) and alters their ratio, increasing infection risk. Immune function recovers within 24 hours post-surgery.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Use of anti-D in immune thrombocytopenic purpura as a means to prevent splenectomy: case reports from two University Hospital Medical Centers.

    Seminars in hematology·2000
    Same author

    Effect of estrogen on nitric oxide and endothelin-1.

    Annals of internal medicine·1998
    Same author

    In situ endothelin in coronary artery disease.

    Angiology·1996
    Same author

    Non-insulin dependent diabetes mellitus and non-Hodgkin's lymphoma. Already reported.

    BMJ (Clinical research ed.)·1995
    Same author

    Interleukin-1 alpha as a factor in occlusive vascular disease.

    American journal of clinical pathology·1992
    Same author

    Development of 5q- myelodysplasia in a patient with sarcoidosis.

    American journal of hematology·1990

    Area of Science:

    • Immunology
    • Cardiovascular Surgery

    Background:

    • Open heart surgery involves cardiopulmonary bypass (CPB).
    • CPB can impact immune function.
    • Lymphocyte subsets play a crucial role in immune response.

    Purpose of the Study:

    • To quantify peripheral blood lymphocyte subsets in patients undergoing open heart surgery.
    • To investigate the impact of CPB on T4 (helper) and T8 (cytotoxic) lymphocyte subsets.
    • To assess the temporal changes in lymphocyte subsets and the T4/T8 ratio post-surgery.

    Main Methods:

    • Immunofluorescence was used to quantify lymphocyte subsets in peripheral blood.
    • Nine patients with coronary artery, valvular, and congenital heart disease were studied.
    • Measurements were taken preoperatively, 2 hours post-CPB, and 24 hours post-surgery.

    Main Results:

    • All patients exhibited absolute lymphopenia and reduced T4 lymphocytes 2 hours post-CPB.
    • A significant reversal of the T4/T8 ratio was observed 2 hours post-CPB.
    • Lymphocyte subsets and the T4/T8 ratio returned to normal 24 hours post-surgery.

    Conclusions:

    • CPB induces transient immune dysfunction, characterized by lymphopenia and altered T4/T8 ratio.
    • This temporary immune suppression may increase susceptibility to postoperative infections.
    • Potential implications include concerns about viral transmission and acquired immune deficiency syndrome post-CPB.

    Related Experiment Videos