Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.4K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Erratum: NanorulerQA: quantitative quality analysis of dual-color DNA nanorulers via single molecule photobleaching counting and spatio-temporal colocalization: erratum.

Biomedical optics express·2026
Same author

Preclinical characterization of HEC116094, an oral inhibitor of the influenza A virus polymerase PB2 subunit.

Emerging microbes & infections·2026
Same author

Digital Analysis of Upper Airway Morphology in Obstructive Sleep Apnea Syndrome Using Cone Beam Computed Tomography.

International dental journal·2026
Same author

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same author

High-Throughput Recognition and Detection of Multiclass Antibiotics Based on Fluorescence-Ultraviolet Dual-Signal CQDs Sensor Array.

Analytical chemistry·2026
Same author

Dual-color single-molecule localization microscopy with a sub-nanometer channel misalignment and sub-1% color crosstalk.

Optics express·2026

Related Experiment Video

Updated: Sep 6, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

PCIe-based FPGA-GPU heterogeneous computation for real-time multi-emitter fitting in super-resolution localization

Dan Gui1,2,3, Yunjiu Chen1, Weibing Kuang1

  • 1Britton Chance Center and MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China.

Biomedical Optics Express
|July 5, 2022
PubMed
Summary

This study introduces a novel all-in-one heterogeneous computing platform (AIO-HCP) enabling real-time multi-emitter fitting for super-resolution microscopy. The AIO-STORM method achieves high-density, large-field-of-view imaging without sacrificing quality.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.0K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.6K

Related Experiment Videos

Last Updated: Sep 6, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.0K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

11.6K

Area of Science:

  • Biophysics
  • Computational Imaging
  • Microscopy Technology

Background:

  • Real-time multi-emitter fitting is crucial for super-resolution localization microscopy (SRLM).
  • High emitter densities (>0.6 mol/µm²) challenge current GPU-accelerated SRLM systems due to computational demands.
  • Existing methods struggle with real-time processing for large fields of view and high densities.

Purpose of the Study:

  • To develop a high-throughput computing platform for real-time multi-emitter fitting in SRLM.
  • To overcome data throughput limitations in heterogeneous computing for microscopy.
  • To enable advanced SRLM applications like dynamic imaging and multi-scale stitching.

Main Methods:

  • Implemented a Peripheral Component Interconnect Express (PCIe)-based all-in-one heterogeneous computing platform (AIO-HCP).
  • Utilized task parallelism for direct and simultaneous data interaction between Field Programmable Gate Arrays (FPGAs) and Graphics Processing Units (GPUs).
  • Developed the AIO-STORM fitting algorithm leveraging big data stream parallel scheduling.

Main Results:

  • Achieved a data throughput of up to ~1.561 GB/s between FPGA and GPU on the AIO-HCP.
  • Demonstrated real-time image processing for 100 µm × 100 µm fields of view with 10 ms exposure and 5.5 mol/µm² density using AIO-STORM.
  • Maintained image quality without sacrifice at high emitter densities and large fields of view.

Conclusions:

  • The PCIe-based AIO-HCP overcomes data throughput bottlenecks in heterogeneous computing for SRLM.
  • AIO-STORM enables unprecedented real-time processing capabilities for high-density, large-field-of-view super-resolution microscopy.
  • This platform facilitates advanced SRLM techniques, including multi-scale image stitching.