Aluminum Oxide-Coated Particle Differentiation Employing Supervised Machine Learning and Impedance Cytometry

This article uses a supervised machine learning (ML) system for identifying groups of nanoparticles coated with metal oxides of varying thicknesses using a microfluidic impedance cytometer. These particles generate unique impedance signatures when probed with a multifrequency electric field and finds applications in enabling many multiplexed biosensing technologies. However, current experimental and data processing techniques are unable to sensitively differentiate different metal oxide coated particle types. Here, we employ various machine learning models and collect multiple particle metrics measured. In reported experiments, a 75% accuracy was determined to separate aluminum oxide coated (10nm and 30nm), which is significantly greater than observing only univariate data between different microparticle types. This approach will enable ML models to differentiate such particles with greater accuracies.