Stability of vancomycin hydrochloride in various concentrations of dextrose injection

The stability of vancomycin hydrochloride in plastic syringes containing high concentrations of dextrose injection after storage for 24 hours in a refrigerator followed by storage for two hours at room temperature was studied. Vancomycin hydrochloride was reconstituted with sterile water for injection to a concentration of 50 mg/mL. One-milliliter samples were added to 9 mL of various concentrations of dextrose injection (5, 10, 15, 20, 25, and 30%) in 10-mL plastic syringes. Ten syringes of each concentration were stored at 4 degrees C for 24 hours. At various storage times, samples were assayed in triplicate for vancomycin using high-performance liquid chromatography. After 24 hours, the syringes were removed from the refrigerator, and the vancomycin concentration was determined after storage for two hours at room temperature. Percent change in vancomycin concentration during storage for 24 hours was less than 6% in all cases except for 5% dextrose injection at 4 and 24 hours. Vancomycin concentration did not change (percent change 0.7-5%) during storage for two hours at room temperature. Vancomycin hydrochloride is stable in various concentrations of dextrose injection when stored in plastic syringes for 24 hours in the refrigerator followed by two hours at room temperature.