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Cat8 Response to Nutritional Changes and Interaction With Ehrlich Pathway Related Factors
Zhengda Du1,2, Hong Deng1,2, Yanfei Cheng1
1CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Frontiers in Microbiology
|July 5, 2022
Summary
The transcription factor Cat8 regulates both carbon and nitrogen metabolism in yeast. This study reveals Cat8
Area of Science:
- * Molecular and Cellular Biology
- * Yeast Genetics and Metabolism
Background:
- * Cat8 (carbon catabolite 8) is a known regulator of non-fermentative carbon source utilization in *Saccharomyces cerevisiae*.
- * Previous research suggested a role for Cat8 in nitrogen metabolism, but the underlying mechanisms remained unclear.
Purpose of the Study:
- * To elucidate the regulatory mechanism by which Cat8 influences nitrogen metabolism.
- * To investigate the functional relationship between Cat8, Snf1 kinase, and other transcriptional regulators in nutrient metabolism.
Main Methods:
- * Analysis of Cat8 nuclear localization and regulatory activity.
- * Comparison of gene expression profiles and Cat8 target gene binding after Cat8 overexpression.
- * Investigation of interactions between Cat8, Aro80, and Gat1.
Main Results:
- * Cat8 function was shown to depend on Snf1 kinase.
- * Overexpression of Cat8 enhanced transcription of genes involved in the Ehrlich pathway and related nitrogen metabolism factors.
- * Cat8 was found to interact with Aro80 and Gat1 to co-regulate *ARO10* transcription.
- * Cat8 demonstrated participation in regulating nitrogen metabolism.
Conclusions:
- * Cat8 acts as a global transcription factor responding to nutritional cues.
- * Cat8 regulates both carbon and nitrogen utilization in yeast.
- * Findings provide new insights into yeast cell nutrient metabolism networks.
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