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Correlation between Platelet miRNA Expression and Coagulation Function in Children with Severe Pneumonia
Mingming Hao1, Jing Guo1, Jinying Lu1
1Department of Pediatrics, Hengshui People's Hospital, Hengshui, Hebei, China.
Insights
Platelet microRNAs miR-223 and miR-192 levels correlate with coagulation function in children with severe pneumonia (SP). These findings offer insights for clinical management and prognosis in pediatric SP cases.
Area of Science:
- Pediatric Medicine
- Molecular Biology
- Hematology
Background:
- Severe pneumonia (SP) in children presents significant clinical challenges.
- Understanding the molecular mechanisms and biomarkers associated with SP is crucial for effective management.
- Platelet microRNAs (miRNAs) are increasingly recognized for their roles in various pathological conditions.
Purpose of the Study:
- To investigate the relationship between platelet miRNA expression levels and severe pneumonia in children.
- To explore the correlation between specific platelet miRNAs (miR-223 and miR-192) and coagulation function parameters in pediatric SP.
Main Methods:
- A randomized controlled trial involving 129 children hospitalized with SP and 129 healthy controls.
- Detection of platelet miR-223 and miR-192 expression using real-time quantitative polymerase chain reaction (qPCR).
- Assessment of coagulation function parameters: prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen (FIB).
- Pearson correlation analysis to evaluate the association between miRNA levels and coagulation markers.
Main Results:
- Children with SP exhibited significantly higher miR-223 and lower miR-192 levels compared to healthy controls (P < 0.05).
- Progressive increases in miR-223 and decreases in miR-192 were observed with increasing severity of infection (mild, moderate, severe).
- SP patients showed significantly altered coagulation parameters: lower PT and APTT, and higher FIB (P < 0.05).
- miR-223 positively correlated with PT and APTT, and negatively with FIB; miR-192 showed inverse correlations with these parameters.
Conclusions:
- Platelet miR-223 and miR-192 levels serve as potential biomarkers reflecting coagulation function in children with severe pneumonia.
- These miRNAs can provide a valuable reference for clinical guidance, treatment strategies, and prognosis assessment in pediatric SP.
Objective:
To investigate the relationship between expression levels of platelet miRNAs and severe pneumonia (SP) in children.
Methods:
A randomized controlled trial was conducted in 129 children with SP hospitalized from May 2018 to May 2020. All children joined the study group and were divided into the mild infection group, moderate infection group, and severe infection group according to the diagnostic criteria, 43 cases in each group. Besides, 129 healthy children were selected as the control group. The expression levels of platelet miR-223 and miR-192 were detected by real-time quantitative polymerase chain reaction (qPCR). The prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen (FIB) were detected by the Sysmex CA-1500 System (Sysmex Corporation, Japan). The Pearson analysis was conducted to evaluate the correlation between coagulation function and the levels of miR-223 and miR-192.
Results:
Compared with the control group, miR-223 in the study group was significantly higher and miR-192 was significantly lower (P < 0.05). Compared with the mild infection group, miR-223 was significantly higher and miR-192 was significantly lower in the moderate infection group and severe infection group (P < 0.05). Compared with the control group, PT and APTT were significantly lower and FIB was significantly higher in the study group (P < 0.05). Pearson correlation analysis revealed that miR-223 was positively correlated with PT and APTT (P < 0.05) and negatively correlated with FIB (P < 0.05); miR-192 was negatively correlated with PT and APTT (P < 0.05) and positively correlated with FIB (P < 0.05).
Conclusion:
miR-223 and miR-192 can reflect coagulation function in children with SP, which can provide a certain reference basis for clinical guidance and treatment and prognosis.
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