Related Experiment Video
Updated: Sep 5, 2025

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands
Published on: July 26, 2014
Comparison of virtual microscopy and real microscopy for learning oral histology laboratory course among dental
Yu-Hsueh Wu1,2, Chun-Pin Chiang3,4,5
1Department of Stomatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Background/Purpose:
Virtual microscopy has been used for learning and teaching histology and pathology laboratory course for more than 10 years. This study aimed to compare the learning outcome of virtual microscopy with that of real light microscopy in oral histology laboratory course among dental students.
Materials And Methods:
The study included twenty-three undergraduate dental students who took the oral histology laboratory course. The evaluation methods of the study were divided into two parts: questionnaire survey (6 questions) and histological diagnosis ability assessment tests. The data were collected and analyzed statistically.
Results:
Dental students showed a significantly higher acceptance rate of virtual microscopy than that of real light microscopy for learning oral histology laboratory course (all P-values < 0.01). Moreover, dental students using virtual microscopy with digitized virtual slides for learning oral histology laboratory course possessed significantly better histological diagnosis ability than those using real light microscopy with traditional glass slides for learning oral histology laboratory course (P < 0.001).
Conclusion:
Virtual microscopy with digitized virtual slides seems to be a great method to learn oral histology laboratory course. Based on the results of our study, we believe that the virtual microscopy with digitized virtual slides may gradually replace the real light microscopy with traditional glass slides for learning and teaching oral histology laboratory course. We foresee that virtual microscopy with digitized microscopic images will be extensively applied in many fields in the near future.
Related Concept Videos
Two-Dimensional Microscopy in Microbiology
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Three-Dimensional Microscopy in Microbiology
Overview of Microscopy Techniques
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Overview of Electron Microscopy

