Study on the Correlation between Interleukin-27 and CXCL10 in Pulmonary Tuberculosis

Area of Science:

• * Immunology
• * Molecular Biology
• * Bioinformatics

Background:

• * Pulmonary tuberculosis (TB) is a significant global health challenge.
• * Understanding the molecular mechanisms and identifying biomarkers for TB is crucial for diagnosis and treatment.

Purpose of the Study:

• * To investigate the correlation between interleukin-27 (IL-27) and CXCL10, along with other cytokines, in pulmonary tuberculosis.
• * To explore related microRNAs (miRNAs) through bioinformatics analysis.

Main Methods:

• * Immunohistochemical staining and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were employed.
• * Expression levels of IL-27, CXCL9, CXCL10, and CXCL11 were assessed in lesion tissues and peripheral blood of TB patients and healthy controls.
• * Key miRNAs were predicted using bioinformatics and their expression verified via qRT-PCR.

Main Results:

• * Significantly increased expression of IL-27, CXCL9, CXCL10, and CXCL11 was observed in TB patients.
• * A significant positive correlation was found between IL-27 and CXCL10 (r = 0.68).
• * has-let-7b-5p was significantly downregulated, while has-miR-30a-3p was significantly upregulated and associated with IL-27 and CXCL10.

Conclusions:

• * IL-27 and CXCL10 are significantly interrelated in the context of pulmonary tuberculosis.
• * The identified miRNAs, specifically has-let-7b-5p and has-miR-30a-3p, show a relationship with IL-27 and CXCL10.
• * These findings provide a foundation for developing novel biomarkers for tuberculosis detection and management.