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Genetic mutation of TRPV2 induces anxiety by decreasing GABA-B R2 expression in hippocampus
Gaoyan Liu1, Yamei Liu1, Bowen Niu2
1School of Life Sciences, Shanghai University, Shanghai, 200444, China.
Abstract:
Transient receptor potential vanillic acid 2 (TRPV2) are well recognized for their contributions to neuronal development, cardiac function, immunity and cancer. However, the precise roles for this thermo TRPchannels in neurological disorder remain unknown. In this study, we employed the CRISPR/Cas9 system to generate genetic mutations of TRPV2. Genetic mutation of TRPV2 resulted in autistic-like phenotypes in mice accompanied with the disordered electrical signals recorded by multi-channels in vivo. To determine possible molecular mechanisms, western blotting was further used to assess the possible involvement of several autism-related proteins. The significantly decreased expression of the R2 subunit of the GABA-B receptor in the hippocampus was observed. Together, our findings suggest that genetic mutation of TRPV2 induces autism-like behavior, results in decreased expression of the R2 subunit of the GABA-B receptor.
Insights
Genetic mutation of Transient Receptor Potential Vanillic Acid 2 (TRPV2) channels in mice caused autistic-like behaviors and reduced GABA-B receptor expression. This research highlights TRPV2
Area of Science:
- Neuroscience
- Genetics
- Molecular Biology
Background:
- Transient Receptor Potential Vanillic Acid 2 (TRPV2) channels are implicated in neuronal development, cardiac function, immunity, and cancer.
- The specific roles of TRPV2 in neurological disorders are not well understood.
Purpose of the Study:
- To investigate the function of TRPV2 in neurological disorders.
- To explore the molecular mechanisms underlying TRPV2's role in autism spectrum disorder (ASD).
Main Methods:
- Utilized the CRISPR/Cas9 system to create genetic mutations in TRPV2.
- Recorded in vivo multi-channel electrical signals in mice.
- Employed western blotting to analyze protein expression, focusing on autism-related proteins.
Main Results:
- TRPV2 genetic mutation induced autistic-like phenotypes in mice.
- Disordered electrical signals were observed in the brains of mutant mice.
- Significantly decreased expression of the R2 subunit of the GABA-B receptor was found in the hippocampus.
Conclusions:
- Genetic disruption of TRPV2 can lead to autism-like behaviors.
- TRPV2 mutation is associated with altered brain electrical activity.
- Reduced GABA-B receptor R2 subunit expression may be a key molecular mechanism linking TRPV2 to autism.
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