Synergistic effects of pesticides and environmental variables on earthworm standard metabolic rate

Endogeic earthworms such as Aporrectodea caliginosa play an essential role in the agroecosystems because of their continuous burrowing and feeding (geophagous) activity, which causes a profound impact on soil texture, organic matter decomposition, soil carbon storage, microbial activity, soil biodiversity, and nutrient cycling. Accordingly, endogeic earthworms are being proposed as suitable candidates for the ecotoxicity assessment of polluted soils. However, terrestrial ecotoxicology has little considered the interactive effects from pollutants and environmental variables (temperature, moisture). We acclimatized A. caliginosa for 90 days to two contrasting temperatures (10 °C and 20 °C) and moistures (25 % and 35 %, w/v) in soils contaminated with 20 mg kg^-1 of chlorpyrifos to examine how these two climate change drivers may modulate the pesticide toxicity. We measured the inhibition of cholinesterase (ChE) activities as indicators of organophosphorus exposure, the standard metabolic rate as an integrative physiological biomarker, and the lipid peroxidation (TBARS) and the total antioxidant capacity (TAC) both as indicators of oxidative stress. The main results were: i) chlorpyrifos strongly inhibited ChE activity (>75 % of controls), demonstrating earthworm bioavailability and acute toxicity at the test concentration; 2) a 50 % mortality and loss of body weight (49 %) were found in the earthworms exposed to the most severe environmental conditions (20 °C, 25 %, and pesticide); 3) this latter experimental group displayed a high SMR, which was concomitant with an increase of the oxidative balance index (TBARS/TAC). We postulated that earthworms acclimatized to stressing environmental conditions experienced a higher pesticide-induced metabolic cost and physiological challenges imposed by adverse environmental conditions.