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Related Experiment Video

Updated: Sep 5, 2025

Chicken Embryo as an In Vivo Model to Revive Viable but Non-Culturable Pathogens
09:34

Chicken Embryo as an In Vivo Model to Revive Viable but Non-Culturable Pathogens

Published on: May 30, 2025

263

A new method to rescue embryos contaminated by bacteria.

Ruiqi Li1,2, Fengjiao Du1,3, Songbang Ou1,3

  • 1Reproductive Medicine Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

F&S Reports
|July 5, 2022
PubMed
Summary

Zona pellucida removal successfully rescued bacterial-contaminated embryos, leading to a healthy pregnancy. This technique offers a viable solution for embryo rescue in assisted reproductive technology.

Keywords:
Bacterial contaminationin vitro fertilizationpregnancyzona pellucida

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Area of Science:

  • Reproductive Biology
  • Microbiology
  • Embryology

Background:

  • Bacterial contamination during in vitro fertilization (IVF) poses a significant challenge, potentially compromising embryo development and viability.
  • Obstruction of fallopian tubes led to a patient's infertility, necessitating IVF treatment.
  • The patient's embryos became contaminated with Klebsiella pneumoniae during the IVF process.

Purpose of the Study:

  • To report a case of successful pregnancy achieved with embryos affected by bacterial contamination.
  • To evaluate the efficacy of zona pellucida removal in rescuing contaminated embryos.

Main Methods:

  • Zona pellucida (ZP) removal using acidic Tyrode's solution was performed on bacterial-contaminated zygotes.
  • ZP-free embryos were cultured in a time-lapse system.
  • A single ZP-free blastocyst was selected for embryo transfer.

Main Results:

  • While washing treatments failed, ZP removal allowed some embryos to develop further.
  • Two of five uncontaminated ZP-free embryos developed into blastocysts.
  • A single intrauterine gestation was confirmed, and the patient reached 30 weeks of pregnancy without intrauterine infection.

Conclusions:

  • Zona pellucida removal is a safe and effective method for rescuing embryos contaminated with bacteria.
  • This intervention can improve the success rates of IVF in cases of bacterial contamination.