Strain-boosted hyperoxic graphene oxide efficiently loading and improving performances of microcystinase

Area of Science:

• Environmental Science
• Materials Science
• Biotechnology

Background:

• Harmful Microcystis blooms (HMBs) and their toxins, microcystins (MCs), pose significant threats to aquatic ecosystems and human health.
• Current methods for MC and HMB removal often lack efficiency or pose environmental risks.

Purpose of the Study:

• To develop an eco-friendly and effective strategy for the simultaneous removal of MCs and HMBs.
• To create a stable composite material using hyperoxic graphene oxides (HGOs) and microcystinase A (MlrA).

Main Methods:

• Fabrication of hyperoxic graphene oxides (HGOs) and their characterization.
• Immobilization of pure microcystinase A (PMlrA) onto HGOs to form HGOs@MlrA composite.
• Evaluation of the composite's performance in removing MCs and HMBs, including its ecological and human safety.

Main Results:

• HGOs demonstrated inherent structural strain effects that enhanced MlrA immobilization.
• HGO5 exhibited high loading capacities for both crude and pure MlrA (1,559 mg·g⁻¹ and 1,659 mg·g⁻¹, respectively).
• The HGO5@MlrA composite effectively removed MCs and HMBs, showing improved safety compared to individual components.

Conclusions:

• HGOs serve as a promising carrier material for enhancing the application of MlrA.
• The developed HGOs@MlrA composite offers an effective and eco-friendly solution for simultaneous MC and HMB removal.
• This strategy holds potential for improving water quality and mitigating risks associated with harmful algal blooms.