Strain-boosted hyperoxic graphene oxide efficiently loading and improving performances of microcystinase

Harmful Microcystis blooms (HMBs) and microcystins (MCs) that are produced by Microcystis seriously threaten water ecosystems and human health. This study demonstrates an eco-friendly strategy for simultaneous removal of MCs and HMBs by adopting unique hyperoxic graphene oxides (HGOs) as carrier and pure microcystinase A (PMlrA) as connecting bridge to form stable HGOs@MlrA composite. After oxidation, HGOs yield inherent structural strain effects for boosting the immobilization of MlrA by material characterization and density functional theory calculations. HGO₅ exhibits higher loading capacities for crude MlrA (1,559 mg·g^-1) and pure MlrA (1,659 mg·g^-1). Moreover, the performances of HGO₅@MlrA composite, including the capability of removing MCs and HMBs, the ecological and human safety compared to MlrA or HGO₅ treatment alone, have been studied. These results indicate that HGO₅ can be used as a promising candidate material to effectively improve the application potential of MlrA in the simultaneous removal of MCs and HMBs.