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Aglycone sterics-selective enzymatic glycan remodeling
Anwen Mao1, Yan Zhang1, Guyu Wang1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Iscience
|July 5, 2022
Summary
Researchers developed a novel method for selective enzymatic glycan remodeling by attaching polymers to enzymes. This technique precisely controls glycan remodeling based on aglycone sterics, enabling applications in glycolipid screening and live-cell studies.
Area of Science:
- Biochemistry and Glycoscience
- Biotechnology and Bioengineering
Background:
- Precision glycan remodeling is crucial for understanding biological events and has biotechnological applications.
- The impact of aglycone sterics on glycan remodeling selectivity has been largely overlooked, limiting applications in complex biological systems.
Purpose of the Study:
- To achieve aglycone sterics-selective enzymatic glycan remodeling.
- To develop a method for differentiating aglycone sterics based on enzyme activity.
- To enable applications such as glycolipid partner screening and glycan remodeling in complex biological environments.
Main Methods:
- Controlled grafting of functional polymers onto glycoenzymes to create enzyme-polymer composites.
- Tuning polymer length to vary substrate permeability and establish an activity pattern-based differentiation strategy for aglycone sterics.
- Orchestrating polymer length adjustment with external cues for multi-faceted regulation of aglycone-steric selectivity.
Main Results:
- Successful demonstration of aglycone sterics-selective enzymatic glycan remodeling.
- Implementation of glycolipid partner screening and selective glycan remodeling in complex biological settings.
- Achieved enhanced glycolipid remodeling and temporal control of glycan remodeling on live cells through polymer length modulation and external cues.
Conclusions:
- The developed enzyme-polymer composites offer a novel strategy for precise, aglycone sterics-selective glycan remodeling.
- This approach overcomes limitations of previous methods and expands the applicability of glycan remodeling in biotechnology and biological research.
- The ability to regulate selectivity with external cues opens new avenues for dynamic control of glycan remodeling in complex systems, including live cells.
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