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Monoclonal antibodies binding data for SARS-CoV-2 proteins.
Nawneet Mishra1, Joan Teyra2, RuthMabel Boytz3
1Department of Pathology and Immunology, Washington University School of Medicine in St Louis, St Louis, MO 63110, U.S.A.
Data in Brief
|July 5, 2022
Summary
Researchers biochemically characterized synthetic monoclonal antibodies for SARS-CoV-2 proteins. These robust antibodies, developed using phage display, show promise for academic and therapeutic applications in understanding the coronavirus.
Area of Science:
- Virology and Immunology
- Biochemistry and Molecular Biology
Background:
- The SARS-CoV-2 pandemic necessitates a deeper understanding of the virus.
- There is a demand for reliable reagents for both academic research and therapeutic development targeting SARS-CoV-2.
Purpose of the Study:
- To provide the biochemical characterization of synthetically developed monoclonal antibodies against SARS-CoV-2 proteins.
- To assess the robustness and binding kinetics of these novel antibodies for potential applications.
Main Methods:
- Selection of antibodies from phage-displayed antibody libraries against immobilized SARS-CoV-2 proteins.
- Utilized Fab-phage ELISA and competitive phage ELISA for clone selection.
- Determined antibody binding kinetics using Bio-Layer Interferometry (BLI) and validated against control proteins.
Main Results:
- Successfully selected and characterized monoclonal antibodies with specific binding to SARS-CoV-2 proteins.
- Demonstrated robust antibody binding through kinetic analysis and specificity testing.
- The developed antibodies showed high affinity and specificity for SARS-CoV-2 targets.
Conclusions:
- The study successfully produced and biochemically characterized synthetic monoclonal antibodies for SARS-CoV-2.
- These antibodies are robust and exhibit specific binding kinetics, making them suitable for further research and development.
- The findings support the utility of these reagents in advancing SARS-CoV-2 research and therapeutic strategies.
