Related Experiment Video
Updated: Sep 5, 2025
In Vitro 3D Cell-Cultured Arterial Models for Studying Vascular Drug Targeting Under Flow
Published on: March 14, 2021
A cadaveric perfused model with antegrade arteriovenous pulsatile circulation: a new tool for teaching endovascular
Ahmad Rifahi1, Robin Chastant1, Catherine Forest-Nault1
1Vascular Surgery Department (Rifahi, Febrer), CIUSSS Nord de l'île, Hôpital du Sacré-Cœur de Montréal; Faculté de médecine (Rifahi, Charbonney, Febrer), Université de Montréal, Montréal, Que.; Department of Thoracic and Vascular Surgery (Chastant), Arnaud de Villeneuve Hospital, Montpellier, France; LifeEngine Technologies Inc. (Forest-Nault, Refet-Mollof, Guy), Montréal, Que.; Department of Anatomy (Charbonney, Bronchti), Université du Québec à Trois-Rivières, Trois-Rivières, Que.
Background:
The benefits of using cadaveric humans in surgical training are well documented, and knowledge of the latest endovascular techniques is essential in the daily practice of vascular surgeons. Our study explores the feasibility of an affordable human cadaveric model with pulsatile and heated antegrade perfusion for reliable and reproducible endovascular or surgical simulation.
Methods:
We undertook cannulation of 7 human cadavers embalmed in a saturated salt solution to create a left-to-right central perfusion with a heated solution, from the ascending thoracic aorta to the right atrium. To that end, we used surgically created carotidojugular and femorofemoral arteriovenous fistulas. Biomedical engineers designed a prototype pump for pulsatile circulation. We monitored invasive blood pressure and temperature. We used this model for training for endovascular thoracic aortic procedures and open vascular surgeries.
Results:
The prototype pump achieved a pulsatile flow rate of 4.7 L/min. Effective cadaveric perfusion was achieved for several hours, not only with an arterioarterial pathway but also with arteriovenous circulation. The arterial pressures and in situ temperatures accurately restored vascular functions for life-like conditions. This new model made it possible to successfully perform thoracic endovascular aortic repair, subclavian artery stenting and simulation of abdominal open vascular trauma management. The saturated salt solution method and a specifically designed pump improved cost competitiveness.
Conclusion:
Endovascular simulation on human cadavers, optimized with the pulsatile and heated perfusion system, can be a dynamic adjunct for surgical training and familiarization with new devices. This reproducible teaching tool could be relevant in all surgery programs.
