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Meriandra dianthera Aqueous Extract and Its Fraction Prevents Blood Coagulation by Specifically Inhibiting the

Filmon Kiflezghi Kiflemariam1, Abiel Ghebrehiwet Tewelde1, Ali Mahmud Hamid1

  • 1Department of Clinical Laboratory Sciences, Asmara College of Health Sciences, Asmara, Eritrea.

Journal of Experimental Pharmacology
|July 6, 2022
PubMed

Insights

Meriandra dianthera leaf extract shows potent in vitro anticoagulant activity, specifically prolonging the intrinsic clotting pathway. This extract also demonstrated significant erythrocyte membrane stabilizing properties, indicating its potential for treating coagulation disorders.

Area of Science:

  • Pharmacology
  • Natural Products Chemistry
  • Cardiovascular Research

Background:

  • Cardiovascular disorders are a leading global cause of mortality.
  • Abnormal blood coagulation is a significant risk factor for cardiovascular diseases.
  • Investigating natural sources for anticoagulant compounds is crucial for developing new therapies.

Purpose of the Study:

  • To evaluate the in vitro anticoagulant activity of Meriandra dianthera crude extract and its fractions.
  • To assess the erythrocyte membrane stabilizing potential of the plant extract.
  • To identify bioactive compounds from Meriandra dianthera for potential use in managing coagulation disorders.

Main Methods:

  • Plant leaves were extracted using a decoction method.
  • Extracts were fractionated using liquid-liquid partitioning.
  • Anticoagulant activity was assessed via prothrombin time (PT) and activated partial thromboplastin time (APTT) assays.
  • Membrane stabilizing activity was determined using hypotonic-induced hemolysis.

Main Results:

  • The crude aqueous leaf extract of Meriandra dianthera significantly prolonged APTT, indicating potent anticoagulant activity.
  • Liquid-liquid fractionation identified the residual aqueous fraction as the primary source of anticoagulant activity.
  • Neither the crude extract nor the aqueous fraction affected the extrinsic coagulation pathway (PT).
  • Both the crude extract and aqueous fraction exhibited significant membrane stabilizing activity.

Conclusions:

  • Meriandra dianthera possesses significant in vitro anticoagulant and membrane stabilizing properties.
  • The aqueous residue fraction is responsible for the observed anticoagulant effects.
  • The plant's leaves represent a promising source of novel bioactive molecules for addressing coagulation-related disorders.
Abstract

Keywords:
APTTMeriandra diantheraanticoagulantblood coagulationfractionationin vitromedicinal plantprothrombin time

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