Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Physical Properties Affecting Solubility02:19

Physical Properties Affecting Solubility

23.3K
Solutions of Gases in Liquids
As for any solution, the solubility of a gas in a liquid is affected by the attractive intermolecular forces between solute and solvent species. Unlike solid and liquid solutes, however, there is no solute-solute intermolecular attraction to overcome when a gaseous solute dissolves in a liquid solvent since the atoms or molecules comprising a gas are far separated and experience negligible interactions. Consequently, solute-solvent interactions are the sole...
23.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Organ-Specific Phytochemical Profiles, Wound-Healing and Hemostatic Activities of <i>Symphytum officinale</i> Aerial Parts and Roots with Differential Pyrrolizidine Alkaloid Content.

Molecules (Basel, Switzerland)·2026
Same author

A comparative evaluation of two clinical decision support systems for safer prescribing in older adults: an exploratory single-patient case study.

Frontiers in pharmacology·2026
Same author

Formulation and evaluation of semi-solid extrusion (SSE) 3D-printed drug preparations using poloxamers and polyethylene oxide as co-printed carrier polymers.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026
Same author

Sustainable Extraction of the Tetrahydropalmatine Alkaloid from <i>Stephania rotunda</i> Lour. Tubers Using Eco-Friendly Solvent Systems.

ACS omega·2025
Same author

<i>Verbena officinalis</i> L. Herb Extract, Its Amino Acid Preparations and 3D-Printed Dosage Forms: Phytochemical, Technological and Pharmacological Research.

Plants (Basel, Switzerland)·2025
Same author

Antimicrobial core-shell nanofiber wound dressing with chloramphenicol-liposomes.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2025
Same journal

Design and evaluation of a pump-free ultrasonic atomization-driven hollow microneedle array for transdermal drug delivery.

International journal of pharmaceutics·2026
Same journal

Predicting milling performance of pharmaceutical crystals from elastic modulus.

International journal of pharmaceutics·2026
Same journal

Lipid digestion- driven drug fate as a key determinant of SNEDDS performance: Mechanistic basis of absorption and in vitro- in vivo disconnect.

International journal of pharmaceutics·2026
Same journal

Redox-responsive nanomedicine beyond glutathione: harnessing reactive oxygen species and emerging endogenous triggers for precision drug delivery.

International journal of pharmaceutics·2026
Same journal

Preventing tablet defects through vacuum-assisted deaeration of a powder bed.

International journal of pharmaceutics·2026
Same journal

Approaches for enhancing bioavailability of macromolecular drugs.

International journal of pharmaceutics·2026
See all related articles

Related Experiment Video

Updated: Sep 5, 2025

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

22.4K

Fast imaging-based single particle analysis method for solubility determination.

Emma Hokkala1, Clare J Strachan1, Mikael Agopov2

  • 1Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland.

International Journal of Pharmaceutics
|July 6, 2022
PubMed
Summary
This summary is machine-generated.

Single particle analysis (SPA) offers a rapid, green method for measuring chemical compound solubility, consuming minimal sample. This image-based technique shows excellent correlation with the gold standard shake-flask method.

Keywords:
DissolutionImage analysisMiniaturizationOptical imagingSingle particleSolubility

More Related Videos

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

8.0K
A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients
11:27

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

Published on: August 9, 2022

2.2K

Related Experiment Videos

Last Updated: Sep 5, 2025

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

22.4K
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

8.0K
A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients
11:27

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

Published on: August 9, 2022

2.2K

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Materials Science

Background:

  • Solubility and dissolution rates are critical physicochemical properties for industrial and research applications.
  • Current methods for solubility measurement often lack accuracy, speed, and environmental sustainability, requiring significant sample quantities.

Purpose of the Study:

  • To validate a novel non-specific, image-based single particle analysis (SPA) method for solubility testing.
  • To compare the SPA method against the established shake-flask (SF) method using UV-Vis spectroscopy.
  • To assess the potential of SPA as a rapid, green, and low-consumable alternative for solubility determination.

Main Methods:

  • Solubility was determined for five biologically active compounds with varying physicochemical properties (pKa, logP) using both SPA and the SF method.
  • UV-Vis spectroscopy was employed for concentration determination in the SF method.
  • Image-based SPA was utilized for direct, non-specific analysis of individual particles.

Main Results:

  • SPA demonstrated excellent linear correlation (R2 = 0.99) with the gold standard shake-flask method for mid-solubility range compounds.
  • SPA exhibited higher variability attributed to individual particle property differences, a factor not captured by traditional bulk methods.
  • Average solubility values obtained via SPA were comparable to those from the SF method.

Conclusions:

  • The single particle analysis (SPA) method shows significant potential as an analytical tool for small-scale solubility studies.
  • SPA offers practical advantages including speed, reduced consumables, and no need for prior compound knowledge compared to the shake-flask method.
  • SPA provides a viable green chemistry approach for characterizing compound solubility.