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Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
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Human ABCE1 exhibits temperature-dependent heterologous co-functionality in S. cerevisiae
1Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
FEBS Open Bio
|July 6, 2022
Summary
Human ABCE1 protein, a ribosome recycling factor, could not fully complement yeast Rli1 knockout at 30°C. However, it showed partial function at 37°C, suggesting temperature-dependent co-functionality.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- ABCE1 protein (Rli1 in Saccharomyces cerevisiae) is a crucial ribosome recycling factor.
- It possesses a unique structure with an N-terminal FeS cluster domain and two ATPase domains.
- Ribosome recycling is essential for protein synthesis and cellular homeostasis.
Purpose of the Study:
- To investigate the heterologous functional complementation of yeast Rli1 by human ABCE1.
- To determine the temperature dependency of human ABCE1 function in a yeast model system.
- To explore the potential co-functionality between human ABCE1 and yeast factors.
Main Methods:
- Heterologous expression of human ABCE1 in Saccharomyces cerevisiae.
- Conditional knockout of the endogenous ABCE1 (Rli1) gene in yeast.
- Growth assays at different temperatures (30°C and 37°C) to assess complementation.
Main Results:
- Human ABCE1 failed to complement the yeast Rli1 knockout at the standard temperature of 30°C.
- Significant, albeit low, yeast growth was observed at a higher temperature of 37°C.
- This indicates a temperature-dependent functional complementation of yeast Rli1 by human ABCE1.
Conclusions:
- Human ABCE1 exhibits temperature-dependent functionality when expressed in yeast.
- The observed complementation suggests potential heterologous co-functionality with yeast translation factors.
- Functional upregulation of human ABCE1 at its optimal temperature may explain the observed growth at 37°C.
Keywords:
ABCE1RLI1Saccharomyces cerevisiaeheterologous functionalityribosome recyclingtemperature-dependencytranslationMore Related Videos
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