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FUCCItrack: An all-in-one software for single cell tracking and cell cycle analysis
Hubert M Taïeb1, Luca Bertinetti1,2, Tom Robinson3
1Department of Biomaterials, Max Planck Institute of Colloids and Interfaces, Potsdam, Germany.
Plos One
|July 6, 2022
Summary
FUCCItrack is a new software for analyzing cell cycle dynamics. It segments, tracks, and visualizes FUCCI-labeled cells, enabling quantitative studies of cell proliferation and morphology.
Area of Science:
- Cell Biology
- Biotechnology
- Bioimaging
Background:
- Single-cell segmentation and tracking are conventional methods.
- Single-cell cycle dynamics analysis is an emerging area of interest.
- Fluorescent ubiquitination-based cell cycle indicator (FUCCI) systems enable real-time quantitative studies.
Purpose of the Study:
- To introduce FUCCItrack, an all-in-one, semi-automated software.
- To enable segmentation, tracking, and visualization of FUCCI-modified cell lines.
- To facilitate quantitative analysis of cell proliferation, migration, and morphology changes in relation to the cell cycle.
Main Methods:
- Development of FUCCItrack software with a user-friendly graphical interface.
- Semi-automated segmentation and tracking of FUCCI-labeled cells.
- Inclusion of features for error identification and manual correction for full analysis control.
Main Results:
- FUCCItrack provides a comprehensive platform for analyzing FUCCI-modified cell lines.
- The software allows for quantitative assessment of both collective cell proliferation and individual cell behaviors.
- Analysis includes cell migration and changes in nuclear or cell morphology over the cell cycle.
Conclusions:
- FUCCItrack offers a powerful tool for researchers studying cell cycle dynamics.
- The software enhances the quantitative analysis of cell proliferation and morphology.
- FUCCItrack supports detailed investigation of single-cell behaviors in real-time.
