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Aging-Associated Changes in Oxidative Stress Negatively Impacts the Urinary Bladder Urothelium
Mathijs M de Rijk1,2, Amanda Wolf-Johnston3, Aura F Kullmann3
1Department of Urology, School for Mental Health and Neuroscience, Faculty of Health, Medicine and Life Sciences, Maastricht University, Maastricht, The Netherlands.
International Neurourology Journal
|July 6, 2022
Summary
Aging increases oxidative stress and reactive oxygen species in the bladder urothelium, potentially causing lower urinary tract symptoms. These findings may lead to new pharmaceutical targets for age-related bladder dysfunction.
Area of Science:
- Urology
- Gerontology
- Cellular Biology
Background:
- Lower urinary tract symptoms (LUTS) increase with age, impacting quality of life.
- The urothelium acts as a barrier and signaling tissue in the bladder.
- Aging causes oxidative stress in the bladder mucosa and urothelium, affecting mitochondrial health.
Purpose of the Study:
- To investigate the impact of aging on urothelial mitochondrial health and bioenergetics.
- To assess age-related changes in oxidative stress and cellular senescence markers in rat urothelium.
Main Methods:
- Collected mucosal tissue from young and aged rats.
- Evaluated senescence and oxidative stress biomarkers (p21-Arc, nitrotyrosine, cytochrome C).
- Assessed urothelial cell reactive oxygen species, mitochondrial membrane potential, bioenergetics, and ATP release.
Main Results:
- Aged rats showed increased senescence and oxidative stress biomarkers.
- Urothelial cells from aged rats had higher reactive oxygen species and lower mitochondrial membrane potential.
- Mitochondrial bioenergetics were significantly reduced in aged urothelial cells.
Conclusions:
- Aging-associated oxidative stress and reactive oxygen species contribute to LUTS.
- Identified mechanisms offer potential pharmaceutical targets for bladder dysfunction in older adults.
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