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The tumor suppressor function of hsa_circ_0006282 in gastric cancer through PTEN/AKT pathway

Zhe Li1, Yi Xie2, Bingxiu Xiao2

  • 1Department of Gastroenterology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, 315020, China.

International Journal of Clinical Oncology
|July 6, 2022
PubMed
Abstract

Insights

Circular RNA hsa_circ_0006282 suppresses gastric cancer growth. Downregulation enhances proliferation and migration, while its overexpression inhibits these processes by regulating the PTEN/AKT pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in cancer development.
  • The specific roles of circRNAs in gastric cancer remain largely unexplored.
  • This study investigates the function of hsa_circ_0006282 in gastric cancer.

Purpose of the Study:

  • To elucidate the role of hsa_circ_0006282 in gastric cancer.
  • To determine the molecular mechanisms underlying hsa_circ_0006282's function.
  • To assess the potential of hsa_circ_0006282 as a therapeutic target.

Main Methods:

  • Quantitative real-time PCR to measure hsa_circ_0006282 levels in gastric cancer cell lines and tissues.
  • RNA interference and plasmid transfection to manipulate hsa_circ_0006282 expression.
  • Cell proliferation, migration, apoptosis assays, and Western blot analysis to evaluate biological effects.

Main Results:

  • Hsa_circ_0006282 was significantly downregulated in gastric cancer tissues and cells.
  • Downregulation of hsa_circ_0006282 promoted cell proliferation, migration, and resistance to apoptosis.
  • Hsa_circ_0006282 regulates the PTEN/AKT signaling pathway by sponging hsa-miR-136-5p.

Conclusions:

  • Hsa_circ_0006282 acts as a tumor suppressor in gastric cancer.
  • It inhibits gastric cancer growth by modulating the PTEN/AKT pathway via hsa-miR-136-5p sponging.
  • Hsa_circ_0006282 represents a potential therapeutic target for gastric cancer treatment.
Keywords:
Biological functionsCircRNAsGastric cancerMiRNAPTEN/AKT signaling pathway

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