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Identification and Characterization of Three Spore Wall Proteins of Enterocytozoon Bieneusi

Xinan Meng1, Haojie Ye1, Ziyu Shang1

  • 1Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.

Frontiers in Cellular and Infection Microbiology
|July 7, 2022
PubMed

Insights

Researchers identified three spore wall proteins (SWPs) in Enterocytozoon bieneusi, a common microsporidian pathogen. A monoclonal antibody against EbSWP1 enabled sensitive and specific detection of E. bieneusi spores in fecal samples.

Area of Science:

  • Veterinary Parasitology
  • Microbiology
  • Immunology

Background:

  • Enterocytozoon bieneusi is a significant microsporidian pathogen affecting both animals and humans.
  • Spore wall proteins (SWPs) are crucial for microsporidian structure and function, but those of E. bieneusi are poorly understood.

Purpose of the Study:

  • To identify and characterize E. bieneusi SWPs.
  • To develop an immunological detection method for E. bieneusi spores.

Main Methods:

  • Bioinformatic analysis of whole genome data to identify SWP sequences.
  • Recombinant expression of E. bieneusi SWPs in Escherichia coli.
  • Generation of monoclonal antibodies (mAbs) against identified SWPs.
  • Direct immunofluorescence assay (DIFA) for detecting E. bieneusi spores in fecal samples.

Main Results:

  • Three E. bieneusi SWP sequences were identified: EbSWP1, EbSWP2 (containing a BAR2 domain), and EbSWP3 (containing a MICSWaP domain).
  • A specific mAb against EbSWP1 was successfully generated without cross-reactivity with other identified SWPs.
  • The developed DIFA demonstrated a sensitivity of 91.4% and specificity of 73.7% for detecting E. bieneusi spores in fecal samples compared to chromotrope 2R staining.

Conclusions:

  • The study successfully identified novel SWPs of E. bieneusi and developed a specific mAb against EbSWP1.
  • The developed direct immunofluorescence assay offers a promising tool for the immunological detection of E. bieneusi infections.
Keywords:
E. bieneusidetectionimmunofluorescencemicrosporidiaspore wall protein

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