Development of Carcinoembryonic Antigen Rapid Detection System Based on Platinum Microelectrode

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Early and sensitive detection of carcinoembryonic antigen (CEA) is crucial for improving colorectal cancer diagnosis.
• Existing diagnostic methods may lack the required sensitivity or speed for effective early detection.

Purpose of the Study:

• To develop a highly sensitive and rapid microsensor for the quantitative detection of CEA in blood.
• To utilize a platinum microelectrode modified with gold nanoparticles and a CEA aptamer for enhanced CEA detection.

Main Methods:

• Fabrication of a platinum microelectrode (PtμE) modified with gold nanoparticles (Au).
• Conjugation of a CEA aptamer onto the PtμEs/Au surface as the recognition element.
• Quantitative analysis of CEA concentration using square wave voltammetry.

Main Results:

• The PtμEs/Au aptasensor demonstrated a linear response to CEA in the range of 1.0 × 10^-11-1.0 × 10^-7 g/ml.
• Achieved a low detection limit of 7.7 × 10^-12 g/ml with excellent linearity (R² = 0.999).
• The aptasensor showed good selectivity against other blood proteins and validated results with clinical samples using minimal blood volume (20 μl).

Conclusions:

• The developed PtμEs/Au aptasensor provides a sensitive, rapid, and cost-effective method for CEA detection in blood.
• This microsensor holds significant potential for clinical diagnosis and early screening of colorectal cancer.