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Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
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Comprehensive RNA-seq reveals molecular changes in kidney malignancy among people living with HIV

Juan Bao1, Jianqing Ye2,3, Jingjing Xu1

  • 1Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Jinshan District, Shanghai 201508, China.

Molecular Therapy. Nucleic Acids
|July 7, 2022
PubMed

Insights

Researchers identified key gene expression changes in kidney cancer among people with HIV (PLWH). These findings in HIV-associated kidney cancer mirror those in the general population, offering new diagnostic and therapeutic insights.

Area of Science:

  • Oncology
  • Virology
  • Genomics

Background:

  • Kidney malignancy awareness is crucial for early diagnosis in patients with HIV infection.
  • Understanding molecular alterations in HIV-associated kidney cancer is vital for improving patient outcomes.

Purpose of the Study:

  • To identify differentially expressed messenger RNAs (mRNAs) in HIV-associated kidney cancer using RNA sequencing.
  • To facilitate early diagnosis and explore novel therapeutic strategies for kidney cancer in people living with HIV (PLWH).

Main Methods:

  • RNA sequencing was employed to analyze differentially expressed mRNAs in HIV-associated kidney cancer.
  • Real-time PCR and immunohistochemical staining were used to validate gene expression levels and protein presence.

Main Results:

  • 2,962 protein-coding transcripts were identified in HIV-associated kidney cancer.
  • KISS1R, CAIX, and NPTX2 mRNA levels were elevated, while UMOD and TMEM213 mRNA levels were decreased.
  • Findings in HIV-associated kidney cancer align with those observed in the general population with renal cell carcinoma.

Conclusions:

  • Identified molecular changes in kidney malignancy provide insights into cancer progression.
  • These discoveries may lead to new therapeutic avenues to reduce mortality in PLWH diagnosed with cancer.
Keywords:
CAIXHIV infectionHIV-associated kidney cancerKISS1RNPTX2RNA-seqbiomarkerkidney malignancy

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